RNA

Part:BBa_K2379002

Designed by: Ramit B   Group: iGEM17_SVCE_CHENNAI   (2017-09-30)


RNA pHmeter with natural scar site

The RNA pHmeter is used for pH-sensitive translational regulation and initiates translation at pH 8.5. It is based on the RNA encoding gene, SraF from E.coli that is a part of the 5' UTR of the alx locus and brings about alx expression only under alkaline conditions.

Usage and Biology

Further characterisation of this part can be found in the link provided below:

BBa_K2379006(BBa_K2379006)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 118


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Categories
Parameters
None